Preoperative Considerations

Fowler's position
Consider Trendelenburg position with head below heart to prevent syncope in susceptible patients

In order to allow for only 1 IV access site, butterfly catheter can be attached to heparin lock or IV extension tubing after Vacutainer collection device is removed. This will then allow an IV fluid line to be attached and used for administration of sedative drugs. Exactly compatibility will vary based on manufacturer.
Alternatively, blood can be drawn directly from a traditional IV catheter if it is first attached to an IV extension line (4" or longer). Extension tubing prevents collapse and dislodgment of IV catheter when negative pressure is applied to draw blood. Blood can be drawn into 10cc syringes and then injected into Vacutainer tubes through rubber access port. Extension tubing can then be attached to IV fluid line and used for administration of sedative drugs.
If using extension tubing, do not forget to prime it with sterile isotonic IV fluid before connecting to a catheter or existing IV line
Make sure centrifuge is powered on and programed to desired settings before drawing blood. Centrifugation should begin as soon as possible after blood is drawn (no more than 2 minutes)
Tubes must be placed into centrifuge in a counterbalanced configuration for proper centrifuge function (i.e. each tube should have another tube of equal weight directly across from it in the centrifuge). If an odd number of PRF tubes are being centrifuged, a tube with 10cc saline can be used for counterbalancing.

Liquid and solid PRF tubes (colors vary by manufacturer)
Butterfly needle with Vacutainer collection attachment
Heparin lock or IV extension tubing (if single venous access site is also used for sedation)
Centrifuge
Scissors
Empty 3cc syringe (1 for each vial of liquid PRF created)
PRF tray and compactor